Part:BBa_K4765021

stem-loop 1, from 10.1021/acssynbio.2c00416

Introduction

The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.^[1].

Usage and Biology

We place the stem-loop at the 3' end of the CDSs to prevent the degradation of mono-cistron mRNA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416

[1] Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416

[1]