Coding

Part:BBa_K4656001:Design

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-07)
Revision as of 09:44, 8 August 2023 by GuXinji (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


TDC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1248
    Illegal BamHI site found at 732
    Illegal BamHI site found at 834
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 1330
    Illegal AgeI site found at 478
    Illegal AgeI site found at 1402
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since TDC is present in many species, it is best to select the sequence of species with high biological activity in the Escherichia coli system(the one used in our project) during the project to solve the problem of low 5-HT production.

Source

The original sequence of tryptophan decarboxylase(TDC) was derived from the genome of the organism Oryza sativa Japonica Group (Japanese rice). Organism: Oryza sativa Japonica Group (Japanese rice)

References