Coding

Part:BBa_K4765903

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-07)
Revision as of 09:59, 7 August 2023 by Siliang Zhan (Talk | contribs)

MysB codon optimized

We introduced a self-assembly synthetic adhesion system by transfecting this bio-brick into *E. Coli*. The bio-brick is composed of a surface display system(intimin) and the coding sequence of an antigen. The surface display system, which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[^1], possess the outer membrane anchoring of the antigen[^2]. The surface-displayed antigen can specifically interact with the nanobody produced by BBk_XXXXXX. In our project, we took full advantage of the Ag-Nb interaction to create a biofilm with a programmable physical structure[^3]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 532


[edit]
Categories
Parameters
None