Coding

Part:BBa_K4765015

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-07-13)
Revision as of 08:00, 5 August 2023 by Siliang Zhan (Talk | contribs)


image demonstration AnAFP is an antifreeze protein derived from *Ammopiptanthus nanus*. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions[1]. We heterologously expressed codon-optimized AnAFP in *Escherichia coli*, endowing it with antifreeze capability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. *Gene*, *539*(1), 132–140.https://doi.org/10.1016/j.gene.2014.01.013
[edit]
Categories
//cds
Parameters
protein