Coding

Part:BBa_K4765015

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-07-13)
Revision as of 07:36, 5 August 2023 by Siliang Zhan (Talk | contribs)


image demonstration

AnAFP is an antifreeze protein derived from *Ammopiptanthus nanus*. This low-temperature-induced protein is predicted to protect the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions. We performed codon optimization of the AnAFP sequence for E.coli K12 to enable its expression in Escherichia coli, resulting in the production of antifreeze effects.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
protein