Plasmid

Part:BBa_K4606000

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-08-04)
Revision as of 05:49, 5 August 2023 by JerryLi (Talk | contribs)


Vector-AmpR

MiR-3074-5p is a kind of microRNA, which can participate in the physiological and pathological activities of cells by regulating the expression of target genes. And in our system, we found that miR-3074-5p targeted SMAD4 gene in chondrocytes and disrupt the homeostasis of chondrocytes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1163
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1163
    Illegal NgoMIV site found at 487
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 279
    Illegal BsaI.rc site found at 2637
    Illegal SapI site found at 1557


[edit]
Categories
Parameters
None