Composite

Part:BBa_K180006:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-16)
Revision as of 07:48, 16 October 2009 by Rich (Talk | contribs) (→‎Design Notes)

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Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1904


Design Notes

Regulated expression of LacI (C0012) and GFP (with LVA degradation tail, K180001) by lux pR (R0062), controlled by presence of AHL-LuxR in the cell.

Source

See relevant basic biobrick parts (R0062, C0012, B0034, K180001, B0015)

References