Composite

Part:BBa_K4727007:Design

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-07-25)
Revision as of 13:44, 25 July 2023 by Registry (Talk | contribs)

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Pf3 Pseudomonas Phage Gene 3 expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 657
    Illegal BsaI site found at 981


Design Notes

An IPTG inducible promoter has been chosen to regulate the metabolic burden of the packaging cell, a strong RBS has been chosen to facilitate the translation of the protein and finally an intrinsic terminator was chosen to be independent from the protein expression of the cell


Source

Pf3 bacteriophage deposited genome NCBI ID: NC_074763 Registty of Standard biological parts

References