Coding

Part:BBa_K4727005:Design

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-07-25)
Revision as of 13:37, 25 July 2023 by Tommasovar (Talk | contribs)


Pf3 Pseudomonas Phage Gene 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 576
    Illegal BsaI site found at 900


Design Notes

Codon optimised with Codonator3000 (iGEM Sydney 2019) for expression in E. coli and for in vitro synthesis, made RFC[10] compatible, added a start codon and stop codon


Source

Pf3 bacteriophage deposited genome NCBI ID: NC_074763

References

[1] CODONATOR 3000 Copyright © 2019, Tristan Ofner, Fahad Ali, Merrie Caruana, Benj Gonzaga, Nathan Hawkins, Isobel McGrath, Emma Todd.