Coding

Part:BBa_K4727004:Design

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-07-24)
Revision as of 20:55, 24 July 2023 by Tommasovar (Talk | contribs)


M13 Gene3 N2 deletion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205


Design Notes

Codon optimised with Codonator3000 (iGEM Sydney 2019) for expression in E. coli and for in vitro synthesis, made RFC[10] compatible, added a start codon and stop codon


Source

M13 bacteriophage genome

References