Plasmid_Backbone

Part:BBa_K4727003

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-07-24)
Revision as of 16:24, 24 July 2023 by Tommasovar (Talk | contribs)


M13 standard phagemid

This part, is a new backbone intended to be useful in the production of M13 bacteriophages carrying a genome of desire. Ita has been developed starting from a commercially available phagemide (pTZ19R) that has been deleted of all the prohibited restriction sites. Following this, BioBrick RFC[10] standard prefix and suffix were added. Any part of interest can now be easily cloned between the prefix and suffx in order to trasduce the cell if interest. It has been developed with the aim of being used together with part BBa_K4727002 or any other M13 helper phage. The phagemid and the helper plasmid must be co-transformed in E. coli, thus allowing the production of phage particles.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 623
    Illegal suffix found at 645
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 623
    Illegal SpeI site found at 646
    Illegal PstI site found at 660
    Illegal NotI site found at 629
    Illegal NotI site found at 653
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 623
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 623
    Illegal suffix found at 646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 623
    Plasmid lacks a suffix.
    Illegal XbaI site found at 638
    Illegal SpeI site found at 646
    Illegal PstI site found at 660
    Illegal NgoMIV site found at 127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2026
    Illegal SapI site found at 943


[edit]
Categories
//chassis/bacteriophage
Parameters
None