Composite

Part:BBa_K4607006

Designed by: Axel Rojero   Group: iGEM23_Tec-Chihuahua   (2023-07-21)
Revision as of 07:29, 21 July 2023 by Registry (Talk | contribs)

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Expression cassette for LysCSA13-ABD protein

This part contains the linear sequence of the LysCSA13-ABD nucleotide optimized for E. coli. It incorporates some of the most efficient biobricks as described bellow: the T7 promoter with LacO regulations BBa_J435350, the medium strength RBS BBa_Z0262, the triple terminator BBa_J435371, and the high copy pUC ori /Kan R backbone BBa_J435330. It also contains the BBa_K4607000 that codifies for the fused endolysin from the CSA13 bacteriophage, with a very efficient catalytic activity, the albumin binding domain that increases its lifetime, and the x6 HisTag for their posterior purification. Additionally, it has a TEV cleavage site for the removal of the purification tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1100
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1100
    Illegal SpeI site found at 127
    Illegal NotI site found at 1242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1100
    Illegal BglII site found at 30
    Illegal BamHI site found at 1094
    Illegal XhoI site found at 1251
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1100
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1100
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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