Coding

Part:BBa_K4607001

Designed by: Axel Rojero   Group: iGEM23_Tec-Chihuahua   (2023-07-21)
Revision as of 06:30, 21 July 2023 by Axelrohz24 (Talk | contribs)


LysK-ABD-SH3B30

Figure 1.LysK-ABD-SH3B30 protein diagram.

This biobrick is a fusion protein based on the endolysin (Lys) from Staphylococcus aureus bacteriophage K[1], the albumin binding domain (ABD) from streptococcal protein G [2], and the SH3 domain from the Streptococcus agalactiae, Streptococcus uberis y S. aureus bacteriophage B30[3]. Its purpose is to recognize the S. aureus, Streptococcus. agalactiae, Streptococcus uberis cell wall, and degrade it. The part is adapted to the Golden Gate cloning method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None