Coding
Part:BBa_K4880000:Design
Designed by: Xinran Xia Group: iGEM23_BASIS-China (2023-07-13)
PaFS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 910
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1059
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We assembled it with Ptrc and Theophylline-dependent riboswitch.
Source
This sequence is derived from the Picea abies genome and it is adapted to the cyanobacteria codon. We took advantage of the publication of Blanc-Garin et al. (2022).