Composite

Part:BBa_K4883010

Designed by: Yuqi Fu   Group: iGEM23_Hangzhou-BioX   (2023-06-29)
Revision as of 06:17, 29 June 2023 by Registry (Talk | contribs)

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Ptef1-RIB1-ERBV-1-RIB7-PTV-ADE4-Tcyc1

Insert two 2A self-cleaving peptides, ERBV-1 and PTV, between RIB1, RIB7, and ADE4. ERBV-1 and PTV help to achieve bicistronic gene expression in Saccharomyces cerevisiae. Use a strong constitutive promoter Ptef1 to overexpress RIB1, RIB7, and ADE4.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1743
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4090
    Illegal BamHI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176
    Illegal BsaI.rc site found at 1675


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