Coding

Part:BBa_K4891004

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)
Revision as of 06:33, 27 June 2023 by Registry (Talk | contribs)

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glf

It is the key part that is responsible for strengthening glucose utilization rate. The phosphotransferase system was replaced by heterogenously introducing glucose facilitator in E. coli ΔptsH ΔptsH.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 155
    Illegal PstI site found at 346
    Illegal PstI site found at 919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 155
    Illegal PstI site found at 346
    Illegal PstI site found at 919
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 155
    Illegal PstI site found at 346
    Illegal PstI site found at 919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 155
    Illegal PstI site found at 346
    Illegal PstI site found at 919
    Illegal AgeI site found at 331
    Illegal AgeI site found at 487
    Illegal AgeI site found at 499
    Illegal AgeI site found at 1210
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None