Composite
Part:BBa_K180003
Designed by: Richard Wilson, Stephanie Chambers Group: iGEM09_Southampton (2009-10-16)
PTAC testing plasmid (GFP) - basic
As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2097
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
control | trp, lac |
direction | forward |
emission | 511 nm |
emit | 511 |
excitation | 501 nm |
excite | 501 |
lum | |
n/a | tac pR testing plasmid (GFP) - basic |
protein | GFPmut3b |
tag | None |