Part:BBa_J435116

pOSIP-TT (TetR, P21)

pOSIP-TT (TetR, P21)

One-Step Cloning and Chromosomal Integration of DNA

Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1125
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 28
  Illegal XbaI site found at 1397
  Illegal XbaI site found at 5490
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal NheI site found at 6090
  Illegal SpeI site found at 1126
  Illegal PstI site found at 1140
  Illegal NotI site found at 1133
  Illegal NotI site found at 5025
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal BglII site found at 469
  Illegal BamHI site found at 22
  Illegal XhoI site found at 1152
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 1126
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 28
  Illegal XbaI site found at 1397
  Illegal XbaI site found at 5490
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 28
  Illegal XbaI site found at 1397
  Illegal XbaI site found at 5490
  Illegal SpeI site found at 1126
  Illegal PstI site found at 1140
  Illegal AgeI site found at 1343
  Illegal AgeI site found at 3052
  Illegal AgeI site found at 4681
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 357
  Illegal BsaI.rc site found at 3197