DNA

Part:BBa_J435400

Designed by: Open Bioeconomy   Group: FreeGenes   (2023-05-09)
Revision as of 10:01, 10 May 2023 by Vinoo (Talk | contribs)

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T4T5_Int_ThiolS

Modified intein, cleavable by addition of thiol-containing molecules such as cysteine, DTT, and beta-mercaptoethanol. Defined as a T4T5 part.

Note: Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.

FLANKED 5' fusion site: TTCG, 3' fusion site: CGGC

Source organism: N/A, Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003461.html

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None