Composite

Part:BBa_J435300

Designed by: Open Bioeconomy   Group: FreeGenes   (2023-05-08)
Revision as of 20:32, 8 May 2023 by Registry (Talk | contribs)

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AF_lacZ pDest


FLANKED 5' fusion site: A, 3' fusion site: GCTT

Source organism: N/A, Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003373.html

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal PstI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal BamHI site found at 229
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 607
    Illegal BsaI.rc site found at 2


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Categories
Parameters
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