Composite
Part:BBa_K225001:Design
Designed by: Nasa SAVORY Group: iGEM09_Tokyo-Nokogen (2009-10-15)
Green light activated actuator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2202
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1564
Illegal BglII site found at 2456 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1117
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (phytochrome from Synechocystis sp PCC 6803) and EnvZ. I mean that I changed the kinase domain of ScaS to EnvZ to make it work in E coli.
Source
ScaS from Synechocysitis sp. PCC 6803