Composite

Part:BBa_K225001:Design

Designed by: Nasa SAVORY   Group: iGEM09_Tokyo-Nokogen   (2009-10-15)
Revision as of 04:30, 16 October 2009 by Registry (Talk | contribs)

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Green light activated actuator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1564
    Illegal BglII site found at 2456
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (phytochrome from Synechocystis sp PCC 6803) and EnvZ. I mean that I changed the kinase domain of ScaS to EnvZ to make it work in E coli.


Source

ScaS from Synechocysitis sp. PCC 6803

References