Coding

Part:BBa_K4227002:Design

Designed by: Yang Chen   Group: iGEM22_ZJUT-China   (2022-09-29)
Revision as of 03:13, 14 October 2022 by Kaga (Talk | contribs) (→‎References)

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Monellin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design a kind of oral probiotics to solve the oral problems, and the monellin is designed as our report module since its sweetness. And in order to make the part work better as a reporter, we choose the single strain monellin which was connect by the glycine instead of the natural double strain monellin for its better stability of heat and PH.

Source

Synthesized by the Tsingke Biotechnology Co., Ltd.

References

[1] Zhongjun Chen, Cai Heng, Zhengying Li, Xinle Liang, Shangguan Xinchen. Expression and secretion of a single-chain sweet protein monellin in Bacillus subtilis by sacB promoter and signal peptide. Applied Microbiology and Biotechnology.2007;6 (73) : 1377-1381.

[2] Sung-Hou Kim, Chui-Hee Kang, Rosalind Kim, Joong Myung Cho, Yong-Beom Lee, Tae-Kyu Lee. Redesigning a sweet protein: increased stability and renaturability. "Protein Engineering, Design and Selection".1989;8 (2) : 571-575.