Composite

Part:BBa_K4297057

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 02:57, 14 October 2022 by GYang1213 (Talk | contribs)

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pYLXP'-loxp-Ura-loxp-ALD2

The aldehyde dehydrogenases ALD2 gene knock out palsmid, which is used for gene ALD2 deletion.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 495
    Illegal XhoI site found at 1421
    Illegal XhoI site found at 1454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1609
    Illegal NgoMIV site found at 3256
    Illegal AgeI site found at 1391
    Illegal AgeI site found at 2274
    Illegal AgeI site found at 2393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 966
    Illegal BsaI site found at 1394
    Illegal BsaI site found at 2277
    Illegal BsaI.rc site found at 978
    Illegal BsaI.rc site found at 1388
    Illegal BsaI.rc site found at 2271
    Illegal BsaI.rc site found at 2712


[edit]
Categories
Parameters
None