Composite

Part:BBa_K4297054

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 02:56, 14 October 2022 by GYang1213 (Talk | contribs)

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PYLXP'-ARO10-ARO8

The plasmid pYLXP' contains the expressiom cassette of gene ARO10 and ARO8, which can catalyze the reactions of L-phenylalanine to phenylacetaldehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5677
    Illegal BglII site found at 5884
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
    Illegal XhoI site found at 6701
    Illegal XhoI site found at 7681
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4945
    Illegal NgoMIV site found at 6706
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
    Illegal AgeI site found at 5535
    Illegal AgeI site found at 6410
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI site found at 6413
    Illegal BsaI site found at 6997
    Illegal BsaI site found at 7410
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 5082
    Illegal BsaI.rc site found at 5817
    Illegal BsaI.rc site found at 6407
    Illegal BsaI.rc site found at 7917


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