Composite

Part:BBa_K4297042

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 02:50, 14 October 2022 by GYang1213 (Talk | contribs)


PYLXP'-PAR1

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR1, which can catalyze the reaction of phenylacetaldehyde to 2-PE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NheI site found at 5520
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4638
    Illegal NgoMIV site found at 4668
    Illegal NgoMIV site found at 4901
    Illegal NgoMIV site found at 5133
    Illegal NgoMIV site found at 5304
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 4767
    Illegal BsaI.rc site found at 5621
    Illegal SapI.rc site found at 5042


[edit]
Categories
Parameters
None