Composite

Part:BBa_K4297033

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)
Revision as of 02:43, 14 October 2022 by GYang1213 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pYLXP'-loxp-Ura-loxp

The plasmid pYLXP' contains the Cre-lox recombination system, which enables gene expression with exceptional spatial (expresses a gene in specific cells, tissues, or entire organisms) and temporal (at specific time points through the application of the Cre-Lox system) control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
    Illegal XhoI site found at 4657
    Illegal XhoI site found at 4690
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4845
    Illegal AgeI site found at 950
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4630
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 5507


[edit]
Categories
Parameters
None