Composite

Part:BBa_K4429002:Design

Designed by: Anish Mulchandani   Group: iGEM22_IISER-Pune2_India   (2022-06-11)
Revision as of 02:38, 14 October 2022 by Anishmulchandani (Talk | contribs) (→‎Design Notes)


Full Length Anti-MBP Recombinant IgY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 739
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 739
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 739
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Construct intended to be produced in a pET-21b vector. Codes for 2 cistrons, for heavy and light chains, each cistron having its own ribosome binding site and both under a single T7 promoter. [1]

Source

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References