Coding

Part:BBa_K4429000:Design

Designed by: Anish Mulchandani   Group: iGEM22_IISER-Pune2_India   (2022-06-10)
Revision as of 02:34, 14 October 2022 by Anishmulchandani (Talk | contribs)


Anti-MBP Recombinant IgY Light Chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensuring the correct V-C region interface for murine and chicken antibody, removing BioBrick Illegal restriction sites


Source

Anti-MBP Variable Light region found from the genetic sequence of anti-MBP IgG expressed in SHuffle E. coli [2]. The constant light region was found in the germline sequence of chickens (through the IMGT database [1]). The assembled coding sequence was codon optimised for E. coli K12.

References

[1] https://www.imgt.org/ligmdb/view?id=X07174 [2] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072