Coding

Part:BBa_K4429001:Design

Designed by: Anish Mulchandani   Group: iGEM22_IISER-Pune2_India   (2022-06-10)
Revision as of 02:27, 14 October 2022 by Anishmulchandani (Talk | contribs) (→‎References)


Anti-MBP Recombinant IgY Heavy Chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 870
    Illegal SapI.rc site found at 1450


Design Notes

Codon optimisation for E. coli K12, removing biobrick illegal restriction sites. Adding polyhistidene tag.

Source

anti-MBP variable region sequence comes from the coding sequence of anti-MBP IgG expressed in SHuffle E. coli in this Robinson et al. 2015 [1].

References

[1] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072