Composite

Part:BBa_K4238003

Designed by: Keita Hoshino   Group: iGEM22_UTokyo   (2022-10-09)
Revision as of 00:53, 14 October 2022 by Asako (Talk | contribs) (→‎Wet lab result)


Act1p+NLS-VP16-EL222+CYC1t
This part is responsible for the acceptance of blue light in yeast cells. EL222 dimerizes under blue light and binds specifically to a particular DNA sequence (C120). NUS Singapore 2022 created Basic Parts by adding the transcription inducers VP16 and NLS to EL222 to promote specific expressions under blue light. We have implemented this part (BBa_K3570021) with the Constitutive promoter ACT1 and the CYC1 terminator in yeast.

Wet lab result

with using BBa_K4238004 as a reporter, we tested how EL222 activates C120-trcuCYC1 promoter under the blue light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 801
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 801
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 801
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 801
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 801
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None