Part:BBa_K4227026
PnlmC-xip-P34-sigma28
Usage and Biology
The CSP is the component stimulating peptides of the S.mutans. When there is CSP be sensed by the comE, the comE will be phosphorylated and start the PnlmC promoter, and the muB will be expressed to decompose the EPS
σs have been divided into four groups based on their phylogenetic relationships and modular structure. σ28, a Group 3 σ, is the most widely distributed alternative σ factor, making it an attractive candidate for study of its promoter recognition. σ28 controls expression of flagella-related genes in all motile Gram-negative and Gram-positive bacteria, and plays a role in development in some non-motile bacteria. We use the σ28 to start the P28 promotor
The xip-P34 is a kind of analogue of the XIP signal peptide
It can be a kind of competitive inhibitors of comX promotor, so we use this to inhibit the EPS foundation
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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