DNA

Part:BBa_K210013

Designed by: Makoto Kashima   Group: iGEM09_Kyoto   (2009-10-15)
Revision as of 17:44, 15 October 2009 by Registry (Talk | contribs)

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LacI binding sites' tandem sequence #700

This is a tandem sequence of lacI binding sites(GAAATGTGAGCGCTCACAACT). In the project "Time bomb" of iGEM Kyoto 2009, this part was used in order to stbilize linear DNA.

It is very difficult to clone this parts because tandem sequence can induce mutation. So, if you want to use this parts, you should cloning this under 32C under which it is liower possible that mutation happens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None