Terminator

Part:BBa_K4390070

Designed by: Zhongyi Liang   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-13)


This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.


Terminator.png

L1U1H08 Synthetic Terminator

  • Synthetic terminator

Secondary Structure

Mfold-K4390070-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Design

A transcription terminator designed for JUMP assembly (Valenzuela-Ortega & French, 2021).

Usage

This terminator was used in following composite parts.

Name Part Number !
Untagged Dou-PETase K4390088
N-terminal Car9-tagged Dou-PETase K4390085
Untagged Tri-PETase K4390089
N-terminal L2NC-tagged Tri-PETase K4390115
N-terminal L2NC-linker-tagged Tri-PETase K4390116
N-terminal Car9-tagged Tri-PETase K4390086
C-terminal L2NC-tagged Tri-PETase K4390082
C-terminal L2NC-linker-tagged Tri-PETase K4390083
C-terminal Car9-tagged Tri-PETase K4390081
Untagged FAST-PETase K4390090
N-terminal L2NC-tagged FAST-PETase K4390113
N-terminal L2NC-linker-tagged FAST-PETase K4390114
N-terminal Car9-tagged FAST-PETase K4390084
C-terminal L2NC-tagged FAST-PETase K4390076
C-terminal L2NC-linker-tagged FAST-PETase K4390077
C-terminal Car9-tagged FAST-PETase K4390075
Untagged MHETase K4390091
N-terminal L2NC-tagged MHETase K4390117
N-terminal L2NC-linker-tagged MHETase K4390118
N-terminal Car9-tagged MHETase K4390087

Reference

Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003.

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