Part:BBa_K4212019
Optimal_RBS
Very strong RBS in B. subtilis, increases expression to 25 folds.
Usage and Biology
Ribosome binds to this place and start translation. Optimal RBS can improve the efficiency.
References
[1] Nowroozi, F.F., Baidoo, E.E.K., Ermakov, S., Redding-Johanson, A.M., Batth, T.S., Petzold, C.J. & Keasling, J.D. (2014) Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly. Applied Microbiology and Biotechnology. 98 (4), 1567–1581. doi:10.1007/s00253-013-5361-4.
[2] McLaughlin, J.R., Murray, C.L. & Rabinowitz, J.C. (1981) Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. Journal of Biological Chemistry. 256 (21), 11283–11291. doi:10.1016/S0021-9258(19)68589-3.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |