Coding

Part:BBa_K4323002

Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong   Group: iGEM22_UManitoba   (2022-09-30)
Revision as of 18:13, 13 October 2022 by Nihnuhc (Talk | contribs)


Truncated FitD

This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in ,i.Escherichia coli,/i.. This part is a different form of FitD in comparison to BBa_K2683006. The composite part BBa_K4323003 includes the necessary components to express truncated FitD toxin. For more information, please visit our Wiki page.

FitD toxicity

Figure 1. The toxicity of Truncated FitD to Dreissena bugensis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 909
    Illegal BamHI site found at 1019
    Illegal BamHI site found at 2568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None