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Part:BBa_K4245136:Design

Designed by: Bria S VarnBuhler, Jared Moon, Sourav Kumar Dey, Jiahui Wu, Samie R Jaffrey, Akshaya Poonepalle, Shivaek Venkateswaran, Varnica Basavaraj, Manaswi Gorle, Sahana Ram Narayanan, Daeun Lee, Janet Standev   Group: iGEM22_Lambert_GA   (2022-10-09)
Revision as of 16:39, 13 October 2022 by Michellejing (Talk | contribs) (→‎Design Notes)

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Design Notes

The Lettuce split aptamer was developed by researchers at the Weill-Cornell Medical College using the SELEX (systematic evolution of ligands by exponential enrichment) process. For the purposes of Lambert iGEM, the sequence complementary to the left portion of the target— the complement of the middle sequence (BBa_K4245131) within the Rolling Circle Product (RCP)— was added to the 3’ end of the Lettuce left sequence.

The experimentation with this version of Lettuce left was not successful, so Lambert iGEM modified the flanking sequence to include one more nucleotide complementary to the target. The modified Lettuce left (BBa_K4245134) was more functional.

Source

The part was acquired as DNA oligos from Integrated DNA Technologies.



Lettuce Left


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]