DNA

Part:BBa_K2935068

Designed by: Haiyong Shu   Group: iGEM19_FAFU-CHINA   (2019-10-16)
Revision as of 14:22, 13 October 2022 by CSWCMrJi (Talk | contribs) (→‎IDH2 Gene)


IDH2

Isocitrate dehydrogenase(IDH) is a key enzyme in the TCA cycle, IDH2 is it’s gene, which can provide reducing power for cell growth, and participate in algal energy metabolism and anabolism.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 718
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 143
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 718
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 718
    Illegal NgoMIV site found at 778
  • 1000
    COMPATIBLE WITH RFC[1000]



Contribution From NNU-China 2022

Group: [ https://2022.igem.wiki/nnu-china/]

Author: Xiaolu Sun

Characterization from iGEM22-NNU-China

IDH2 Gene

        Isocitrate dehydrogenase (IDH) is a key enzyme in the TCA cycle, which can provide reducing power for cell growth, and participate in algal energy metabolism and anabolism[1]. IDH2 is the gene encoded in Y.lipolytica. It was registered in 2019. In order to characterize the effect of the IDH2 gene in lipid synthesis and growth, we constructed a knockout strain po1f ΔylIDH2 using homologous recombination. The YNB medium have been chosen for testing Results showed that the strain po1f ΔylIDH2 can effectively increase the accumulation of biomass and fatty acid content. Among them, the strain po1f ΔylIDH2 biomass and fatty acid content increased by 5.63% and 10.10%, respectively. These results provide references for future iGEM teams to improve biomass and lipid accumulation in Y.lipolytica.

Fig. 1. The Growth and lipid synthesis of strain po1f ΔylIDH2 under different culture conditions. A. Changes of growth curves in two fermentation mediums. B. Changes of fatty acid content in the two fermentation mediums at 120h

Reference

[1] Morgunov, I. G., Solodovnikova, N. Y., Sharyshev, A. A., Kamzolova, S. V., & Finogenova, T. V. (2004). Regulation of NAD+-Dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica. Biochemistry (Moscow), 69(12), 1391-1398.


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