Part:BBa_K4165172

T7 promoter- His tag- WAP-T7 terminator

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), WAP Inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721), The His tag was attached to the WAP inhibitor coding sequence to serve in the purification using NI-NTA column.

Usage and Biology

Human serine protease inhibitor WAP-four disulfide core domain 13 is able to inhibit HtrA1 (BBa_K4165004) through binding with Htra1 through targeting and degrading both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 379
  Illegal AgeI site found at 115
  
• 1000
  COMPATIBLE WITH RFC[1000]

Dry Lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.

                  Figure 1. this figure shows the results from the transcription and translation code showing the 
                     variation of mRNA and protein concentrations with time compared with the wet lab results.