Part:BBa_K4165149

T7 promoter-6x His tag-HtrA1-T7 terminator

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pET22b RBS (BBa_K2924053), 6x His-tag (BBa_K4165020), HtrA1 (BBa_K4165004), and T7 terminator (BBa_K731721),The His tag was attached to the HTRA1 coding sequence to serve in the purification using NI-NTA column.

Usage and Biology

the expression of serine protease HtrA1 which is an ATP-independent protease that can work extra- and intracellularly, It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 181
  
• 1000
  COMPATIBLE WITH RFC[1000]

Dry Lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.

                   Figure 1. this figure shows the results from the transcription and translation code showing the 
                                     variation of mRNA and protein concentrations with time .