Primer

Part:BBa_K4344033

Designed by: Marcel Pott   Group: iGEM22_Heidelberg   (2022-09-29)
Revision as of 13:15, 13 October 2022 by Leuntor (Talk | contribs)

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NotI-HSV-UL19-6xHis-fwd.

forward primer HSV UL19 (Part: K4344020) with NotI restriction site and with additional His-tag for purification

Usage and Biology

1.2 % Analytical of UL19 siRNA target area PCR with primer sets SacI/XhoI and SalI/NotI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None