Regulatory

Part:BBa_K4497027

Designed by: Till Gundlach   Group: iGEM22_Munich   (2022-10-09)
Revision as of 13:11, 13 October 2022 by Rosareithmeir (Talk | contribs)

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Bidirectional TRE-CMVmin Promoter

The Bidirectional TRE-CMVmin Promoter a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate the amount of a protein of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
    Illegal NotI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. https://doi.org/10.1021/sb400128g

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