Coding

Part:BBa_K4497005

Designed by: Till Gundlach   Group: iGEM22_Munich   (2022-10-09)
Revision as of 12:37, 13 October 2022 by Rosareithmeir (Talk | contribs)


Tobacco Etch Virus (TEV) Protease

The Totobacco etch virus (TEV) protease is a useful reagent for cleaving genetically engineered fusion proteins at a specific site. The TEV Protease recognition sequence is gaaaacctgt attttcagat g (Part K4497006).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 385
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 385
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 385
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


==References

Kapust, R. B., Tözsér, J., Fox, J. D., Anderson, D. E., Cherry, S., Copeland, T. D., & Waugh, D. S. (2001). Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Engineering, 14(12), 993–1000. https://doi.org/10.1093/protein/14.12.993

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