Part:BBa_K4165220

Coh2 linked with tau binding peptide (TD28rev) & GSGS linker

Fusion protein consists of Tau binding peptide (TD28rev) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system.

Usage and Biology

Protac system is known to be a promising one for targeting and signaling many diseases and biological application so according to our project aim, we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregation in our updated protac version system "Snitch system". Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

3D Modeling and Ranking

This fused protein was modeled using Modeller software and after running our quality assessment code it got a score of 5 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its decrease in the overall protein stability as compared to other models. This protein was excluded due to its low QA score as compared to other models.

cbeta_deviations	clashscore	molprobity	ramachandran_favored	ramachandran_outliers	Qmean_4	Qmean_6
1	45.96	2.29	98	0.67	-0.54	-0.94

                           Figure 1.: Predicted 3D Structure of COH2_GSGS_TD28rev Visualized by Pymol.