Regulatory

Part:BBa_K4307027

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)
Revision as of 15:54, 12 October 2022 by YuheWang (Talk | contribs)


PmrC

This part is the promoter regulated by PmrB/A two-component system in E.coli. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307028） system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


bba-k4307027-gel.png

               Figure 1:  The construction results of PmrC.


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Categories
Parameters
None