Composite

Part:BBa_K4390035

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-16)
Revision as of 15:49, 12 October 2022 by Dianajorge (Talk | contribs)


SpyTagged His-tagged M. edulis MT

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

SpyTag is a 13 amino acid-long protein that spontaneously reacts with the SpyCatcher protein (12.3 kDa). Together they form an intermolecular isopeptide bond. This provides an alternative to His-tag purification techniques. Insertion of either the SpyTag or the SpyCatcher sequence into a recombinant protein will allow its purification.

This construct is used to express SpyTagged Mytilus edulis Metallothionein, so that we can assemble a SpyTag-SpyCatcher hydrogel.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 212
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
    Illegal PstI site found at 212
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 212
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 212
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None