Part:BBa_K4390025

Si-tag :: Gly-Ser Linker This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is an NO part containing a silica-binding tag and a glycine-serine linker. The silica-binding tag allows for the expressed level 1 construct to bind to silica, while the glycine-serine linker introduces a short unstructured domain, minimising the risk of the silica tag interfering with the activity of other parts. The 2022 Edinburgh-UHAS iGEM team used this part to bind metallothioneins to silica beads. It does not have TTCG at the end to complete the sequence, as this is regenerated by the O part 3' fusion site in JUMP assembly. Add TTCG at the end to use as a normal part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 14
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]