Composite

Part:BBa_K4216030

Designed by: Yves Loiseau--Marchand   Group: iGEM22_Paris_Bettencourt   (2022-10-11)
Revision as of 15:33, 12 October 2022 by Yves LM (Talk | contribs)


pOxyS Marionette

Input

DpB.005 is an Input device that can transcribe an electrical signal into the production of a protein that will act as a signal for our "processors" (BBa_K4216035/6/7). They are can be used to pass on signals to the Processors and downstream our signal processing pipeline. They can also be used as standalone for prototyping and debug of for specific applications

To know more about this part visit: https://2022.igem.wiki/paris-bettencourt/pOxyS

Electrogenetic TU used as a marionette (https://www.nature.com/articles/s41589-018-0168-3), pOxyS is supposed to be activated by a redox reaction itself drived by an electrode working with a potentiostat, if the device is working, it will activate the CDS of C71m(CinR)(=Bba_K4216020), CinR can then activate another promotor (Bba_K4216008).


Design

Here an overview of our design for BBa K4216030:

Dpb 005.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 972
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 772
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None