Regulatory
pBAD

Part:BBa_K206000:Design

Designed by: Amelia Hardjasa   Group: iGEM09_British_Columbia   (2009-10-14)
Revision as of 01:41, 15 October 2009 by Registry (Talk | contribs)

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pBAD strong


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There were no special design considerations.


Source

The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].

References