Regulatory
pBAD

Part:BBa_K206000

Designed by: Amelia Hardjasa   Group: iGEM09_British_Columbia   (2009-10-14)
Revision as of 01:41, 15 October 2009 by Registry (Talk | contribs)

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pBAD strong

pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (BBa_I13453) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
familypBAD promoter
positive_regulators1