Composite

Part:BBa_K4294811

Designed by: Aristotelis Anastopoulos   Group: iGEM22_Athens   (2022-10-12)
Revision as of 15:15, 12 October 2022 by Pantelistra (Talk | contribs)

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TU constitutive LuxR for PFc, PFc+R

Usage and Biology

It can be used in the QS system of LuxI/LuxR. LuxI synthase catalyzes the production of 3-oxohexanoyl-homoserine lactone (OC6 for short), which belongs to a family of QS molecules called N-Acetyl-Homoserin-Lactones (AHLs). An activated bacterial population can express LuxI and the produced OC6 molecules diffuse outside of this bacterial population to activate another one. Inside the second population, OC6 molecules bind to LuxR, a transcriptional regulator that forms its functional dimer after binding with OC6, which activates the Plux promoter and, therefore, the downstream gene.

Design

We used this transcriptional unit in our Positive Feedback constitutive (BBa_K4294803) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805) constructs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None